In 2010, ONT combined two technologies addressing each of the main outstanding problems. The first was an engineered nanopore developed in collaboration with Bayley’s lab that could discriminate between individual DNA bases, solving the resolution issue. The second was a trick to slow the DNA down to detectable speeds, using the familiar DNA polymerase enzyme. Mark Akeson’s lab at UC Santa Cruz had identified a specific polymerase from the bacterial virus ɸ29 that replicated DNA at an ideal speed for detection via nanopore. Template DNA strands were replicated just before entering the nanopore, passing through slowly enough for individual bases’ effect on the electrical current to be detectable and allowing the DNA sequence to be read one base at a time.
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绿色低碳转型成效突出。初步测算，全年全国万元国内生产总值二氧化碳排放[22]比上年下降5.0%。水电、核电、风电、太阳能发电等清洁能源发电量42481亿千瓦时，比上年增长14.4%。新能源汽车产量1652.4万辆，比上年增长25.1%；年末新能源汽车保有量4397万辆，比上年末增加1257万辆。在监测的339个地级及以上城市中，空气质量达标的城市占72.6%。3641个国家地表水考核断面中，水质优良（Ⅰ—Ⅲ类）断面比例为91.4%，Ⅳ类断面比例为6.9%，Ⅴ类断面比例为1.1%，劣Ⅴ类断面比例为0.6%。，更多细节参见Line官方版本下载
Фото: Пелагия Тихонова / РИА Новости